Cohesion is established during DNA replication utilising chromosome associated cohesin rings as well as those loaded de novo onto nascent DNAs

Cohesin is established by two parallel pathways during DNA replication. Chromosome associated cohesin is converted to cohesive structures and this depends on four protein associated with the replisome; Tof1/Csm3, Ctf4 and Chl1 (TCCC). In TCCC mutants, chromosome associated cohesin is evicted from the DNA during S phase. Characterisation of the effect of WT and mutant cohesin complexes and associated factors on loading onto chromosomes in S. cerevisiae, assessed by calibrated ChIP-seq on an Ion Torrent Proton (Life Technologies)