Polyacrylamide beads measured with RT-DC at different flow rates

Polyacrylamide hydrogel beads from the same production batch were measured with real-time deformability cytometry (RT-DC) at different flow rates (0.04, 0.08, and 0.12 µL/s). The beads were designed to have a Young's modulus of approximately 4.3 ± 1.5 kPa. These measurements were used to illustrate the correction of systematic errors for the computation of the Young's modulus in RT-DC (figure 10 in [1]). The reservoir measurement was added for the sake of completeness. The files were converted from the .tdms file format to the .rtdc file format using DCKit 0.10.0 [2] and dclab 0.27.8 [3].<br><br>Before analysis, the data should be filtered for porosity (1.0 &lt; area_ratio &lt; 1.1) and deformation (0 &lt; deform &lt; 0.035). This is necessary, because events were not always detected correctly (incorrect contour data) due to lack of imaging contrast.<br><br><br><b>References:</b><br>[1] Christoph Herold. Mapping of Deformation to Apparent Young’s Modulus in Real-Time Deformability Cytometry. ArXiv e-prints 1704.00572 [cond-mat.soft], 2017. arXiv:1704.00572v1.<br>[2] https://github.com/ZELLMECHANIK-DRESDEN/DCKit<br>[3] https://github.com/ZellMechanik-Dresden/dclab<br><br><br><b>SHA256 sums:</b><br>7c7e262113384020cab970652d62ac1cccc61d10c89969c1f8fa2c55ec933b35 2016-03-02_M3_0.0400uls_22f26fe5.rtdc<br>db5859d009396c697f1087479913443309e39414ab9d4ccafa3097d229e2e31d 2016-03-02_M4_0.0800uls_1e1e8234.rtdc<br>16c7ca085eeae8590ddc3eb75069ac91cb3d5f5e8d3ec32d641aa4f26d09cdd0 2016-03-02_M5_0.1200uls_d387ab27.rtdc<br>379e8d67fe88861fb8170c3f535f4c7ec34cc52a71e12f7c0d41bbf0545e0a02 2016-03-02_M6_reservoir_efe92abf.rtdc