Expression data from inducible ES stable cell line overexpressing specifically the human gene GTF2I

In order to identify the effects of the induction of the gene of interest (GTF2I) on the mouse ES transcriptome, we performed Affymetrix Gene-Chip hybridization experiments for the inducible not-tagged cell line Transcriptome analysis of the inducible transgenic mouse ES cell line overexpressing the human isorofm of the gene GTF2I For the analysis on the GTF2I inducible cell line, total RNA was extracted from three biological replicates grown in medium deprived of Tetracycline for 24 hours; RNA extracted from un-induced clones was used as control.