Raw reads for single strand consensus sequencing analysis of HIV-1 reverse transcriptase error rate using as template synthetic RNA or HIV-1 viral RNA from 8E5 cells

Raw Illumina reads used to evaluate the accuracy of HIV-1 (M/B/BH10) reverse transcriptase (RT) when copying RNA templates containing the HIV-1 protease (PR)-coding sequence. NGS assays based on single-strand consensus sequencing. Libraries were obtained with the following workflow. First, RNA templates were obtained from in vitro transcription with T7 RNA polymerase or from viral RNA produced by 8E5/LAV infected cells. The obtained RNA was reverse transcribed by the HIV-1 (BH10) RT using a primer with a Unique Molecular Identifier (UMI) or barcode of 14 nucleotides. The cDNAs obtained after reverse transcription were amplified by PCR and during this process adapter sequences were incorporated to facilitate subsequent NGS sequencing on an Illumina MiSeqTM Sequencing System.