Dynamic Single Cell Transcriptomics Defines Kidney FGF23/KL Bioactivity and Novel Segment-Specific Inflammatory Targets [scRNA-Seq]

Wild type mice were injected with rFGF23 for 1, 4 and 12h and renal FGF23 bioactivity was determined at single cell resolution. UMAP plots identified distinct epithelial, endothelial, stromal, and immune cell clusters, with differential expressional analysis uniquely tracking FGF23 bioactivity at each time point. Overall design: C57BL/6J male and female mice were purchased from the Jackson Laboratory at 9-weeks of age and housed in the IUSM Laboratory Animal Resource Center (LARC) for 1 week prior to the start of the experiments. At 10-weeks of age, mice were treated with a single intraperitoneal injection of 10 µg/mouse body weight of rhFGF23 (R&D Systems, Inc.) and euthanized at baseline (0h), 1, 4, 12, or 24h. For scRNAseq experiment, male-female kidney pairs were digested, and cells were isolated as previously described.