Expression data from Ctrl and Cko B10 cells in steady state and anti-CD40 stimulstion for 48 h.

Transcriptional repressor Prdm1/Blimp1 is known to play a key role in controlling B ells differentiation and regulating IL-10 production in regulatory T cells. B10 cells is the main IL-10 producing B cells in mouse spleen. We found that B10 cells and IL-10+ B cell levels are increased in Prdm1-deficient mice. Here, we compared the gene expression profiles of B10 cells from Prdm1-deficient mice (Cko) and its control littermate mice (Ctrl) in steady state and stimulated with anti-CD40 antibody for 48 h. To understand the genes involved in regulating the production of IL-10 by B10 cells, sorted B10 cells were stimulated with anti-CD40 for 48 hrs.