Enhanced Separation of Intact Proteins and Proteoforms by CZE-MS Using Sulfobetaine-Modified Poly(α-L-lysine)-Based Multilayer Coatings for EOF Adjustment

Capillary electrophoresis is a valuable separation technique for proteoform analysis. Here, capillary coatings with different EOF strengths are used to increase the resolution of proteoforms for CE-MS/MS analysis.