Genome sequences of Chlorella sorokiniana UTEX 1602 and Micractinium conductrix SAG 241.80: implications to maltose excretion by a green alga

Micractinium conductrix SAG 241.80 secretes sugars (especially maltose) at pH 5.7 while very little sugar is found at pH 7.6. Another algae, Chlorella sorokiniana does not secrete sugars at either pH. We evaluated the transcriptional profiles of both species of algae at pH 5.7 and 7.6 to make comparisons in the regulation of potential genes involved in these pathways. Overall design: Micractinium conductrix was grown in modified freshwater SAG medium and Chlorella sorokiniana was grown in a modified freshwater medium and both were grown at both pH 5.7 and pH 7.6. All samples were harvested at similar times and total RNA was extracted. Libraries were constructed and sequenced using HiSeq 2500 Illumina sequencing technique.