RNA-seq analysis in mouse cortical extracts with deletion of STI1 TPR1 domain

Long RNA-sequencing was performed on 5 cortex samples from STI1 wt/wt and 5 STI1 dTPR/dTPR mice. RIN values for these samples ranged between 8.2 and 8.7. Illumina sequencing yielded an average of 797,963 reads per sample, with 93.69% reads mapping rate to the mouse genome. We first verified the complete absence of STI1 exons 2 and 3 in the dTPR/dTPR samples compared to the wt/wt control tissues where these axons were both expressed. Principal component analysis and sample distance matrix analyses did not segregate between the two groups, indicating minimal differences between them. Furthermore, thorough RNA sequencing analysis did not reveal any significant changes in transcripts passing FDR correction (padj<0.05). Briefly, there were 85 gene transcripts (representing 18 unique genes) that passed p.value<0.05 and whose levels were changed by more than 1.5 fold increase (or 0.75 decrease) Examination of transcriptome changes in control and transgenic mice lacking STI1-TPR1 domain