BMP gradient along the intestinal villus axis controls zonated enterocyte and goblet cell states. BMP gradient along the intestinal villus axis controls zonated enterocyte and goblet cell states

Single cell and bulk RNA sequencing was performed from human intestinal organoids differentiated for 5 days in the presence or absence of BMP-2 and BMP-4 ligands. Overall design: Single cell and bulk RNA sequencing was performed from human intestinal organoids differentiated for 5 days in the presence or absence of BMP-2 and BMP-4 ligands. For in vivo validation, intestines of wildtype and BMPR1a knockout mice were subjected to bulk RNA sequencing.