TAT.ARC protein administration in the contralateral ventricle decreased lesion volume in subregions of the ischemic territory after 24 h and 72 h after 60 min middle cerebral artery occlusion

Stroke volume and regional stroke volume in 2 mm sagital slices used for the biochemical assays were analyzed using Analyze 5.0 and Image J software. For stroke volumetry hyperintense areas of ischemic tissue in T2-weighted images were assigned with a region of interest tool (ROI). This enabled a threshold-based segmentation which was performed by connecting all pixels within a specified threshold range around the selected seed pixel and resulted in a 3D object map of the whole stroke region. The total volume of the whole object map was calculated automatically. To calculate the regional ischemic lesion volumes that would correspond to slices prepared for immunoblots, axial MRI images were divided with ImageJ into 3 equal slices from medial to lateral. The volumes of hyperintense areas in each brain section were calculated by Analyze 5.0 as described above. Brain parenchyma were cropped for presentation purposes. after 60 min MCAo and quantified