Rho family siRNA screen.

(Sheet 1) Raw data from siRNA screen. Column B indicates the position of the well within the 384 screening plate and the corresponding gene name is found in column C. “#N/A” indicates wells containing siRNA buffer alone and were used to calculate average foci size for mock condition. Blank wells in column C indicate wells located on the edge of the plate and were thus not included in the analysis due to potential edge effect. For each replicate, wild type and ipgB1 averages and standard deviations are calculated as well as lower and upper cutoff scores (1.5 standard deviations below or above the average, respectively). The median foci size per well is shown as well as the corresponding z-score. Wells that fall below lower cutoff or above upper cutoff are highlighted in green and red, respectively. (Sheet 2) Overview of screening data. Each gene name is displayed in column C. The “hit” criteria of how many duplexes were found (out of 4) per replicate and the number of replicates that this result must repeat (out of 4) are indicated in red and orange text, respectively. For wild type and ipgB1, the # of duplexes that resulted in decreased spreading below lower cutoff or increased spreading above upper cutoff for each replicate are shown. Columns A and B indicate the number of replicates in which the “hit” was found. Values for “hits” that decreased or increased spreading are highlighted in green and red, respectively. (XLSX)