Host switching is the main driver of coevolution between Hepatozoon parasites and their vertebrate hosts

Data and scripts used for running all analyses is provided (parasite and host phylogenetic trees constructed with Bayesian inference, binary matrix in text format coding the host parasite associations and R script to implement PACo and ParaFit). We reconstructed the host and parasite phylogenies using 18S rDNA and cytB sequences, respectively. Subsequent analyses were stratified according to host vertebrate orders, Carnivora, Rodentia and Squamata, and the corresponding sequences of their Hepatozoon parasites. Then, Procrustean Approach to Cophylogeny (PACo) and ParaFit were employed to assess their global cophylogenetic relationships. In addition, eMPRess was used to estimate the most probable co-evolutionary events, such as host switch, duplication, sorting or cospeciation, accounting for the shared evolutionary history of Hepatozoon spp. and their vertebrate hosts.