Protein binding to PDCD4 promoter X-box in Nutlin-3a treated U2OS cells
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https://www.omicsdi.org/dataset/pride/PXD020932
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资源简介：
Human PDCD4 wild-type (wt) promoter fragments were amplified from U2OS genomic DNA and X-box deletion mutants (mut) were generated using the QuikChange site-directed mutagenesis kit (Agilent Technologies). DNA probes for affinity purification with the same sequences were obtained by PCR using a biotinylated primer for labeling the 3' end (Thermo Fisher Scientific). DNA affinity purification with nuclear extracts from Nutlin-3a treated U2OS cells.
创建时间：
2024-08-08
