mRNA sequencing of Yarrowia lipolytica strains

mRNA sequencing of the wild type Yarrowia lipolytica 28849 and evolved isolated strains (YLE111, YLE155, YLE198, YLE469, YLE471) of the former, deriving after adaptive laboratory evolution in a synthetic medium following two strategies: 1) gradual increase of pure glycerol concentration as a stressing factor (9-20% v/v) and 2) random chemical mutagenesis with ethyl methanesulfonate (EMS) prior to gradually increasing concentration of pure glycerol as a stressing factor (9-20% v/v). Evolved strains were isolated after 520 generations.