原始数据TMYC-2025-0066.xlsx

The basidiomycete <i>Sporisorium scitamineum</i> causes sugarcane smut disease, leading to a significant impairment in the quality and yield of sugarcane. Polyamines affect the intracellular redox balance, which is important for the pathogenic development of <i>S. scitamineum</i>, including sexual mating, dikaryotic hyphae growth, and host invasion. In the present study, we identified a polyamine transporter, SsTPO1, in <i>S. scitamineum</i>. Deletion or overexpression of <i>SsTPO1</i> led to the disruption of intracellular levels of putrescine, spermidine, and spermine in opposite directions. SsTPO1-based polyamine homeostasis contributes to dikaryotic hyphal growth and host invasion after sexual mating, likely by affecting the cAMP-PKA signaling pathway and collaboratively regulating redox homeostasis. Environmental pH affected SsTPO1-mediated polyamine transport, so <i>S. scitamineum</i> hyphae growth favored neutral to alkaline environments rather than acidic conditions. The SsTPO1-based polyamine transport also participates in stress resistance and autophagy induction. In summary, our work revealed that SsTPO1 facilitates polyamine transport to regulate cell growth, differentiation, and fungal pathogenicity in <i>S. scitamineum.</i>