Analysis of cell surface markers on macrophage subsets.

The expression of the indicated markers was analyzed by FACS on IL-34-Mφ, M-CSF-Mφ and GM-CSF-Mφ, stimulated or not for 48 h with 200 ng/ml LPS. Results are expressed as mean fluorescence intensity values (after subtraction of the MFI obtained with the isotype control mAb) and are representative of 4 separate experiments. +++++ indicates MFI greater than 1000; ++++, MFI greater than 400 and less than 1000; +++, MFI greater than 200 and less than 400; ++, MFI greater than 100 and less than 200; +, MFI greater than 20 and less than 100 and, − indicates MFI less than 20.