Bulk FAST5 file for 16S Oxford Nanopore Barcoded Sequencing

Real-time taxonomic classification of nanopore amplicon sequencing data enables rapid insights into microbial communities, with applications in clinical diagnostics, environmental monitoring, and outbreak surveillance. However, bridging the gap between long-read data and interpretable results often requires specialized bioinformatics expertise, highlighting the need for integrated, user-friendly software that combines live data acquisition with downstream microbiome analysis. To address this need, we developed NANOTAXI, a fully automated Shiny-based graphical user interface (GUI) for classifying barcoded 16S rRNA gene sequences generated by Oxford Nanopore sequencing. NANOTAXI supports four taxonomic classifiers integrated with five reference databases, offering flexible classification strategies tailored to user requirements and computational resources. The dataset accompanying this submission comprises a bulk FAST5 file from a Nanopore sequencing run of barcoded 16S rRNA genes. This bulk FAST5 file enables users to replay the sequencing run using Oxford Nanopore Technologies' MinKNOW software, allowing the testing of novel methods or analytical techniques without performing additional sequencing experiments, thereby saving both time and resources.