Plasma-specific microRNA responses in rats treated with acute toxicity doses of aristolochic acid I

In the present study, goal was to scan the potential biomarker for acute kidney injury induced by aristolochic acid I (AAI).We utilized the microarry analysis to investigate the microRNA (miRNA) expression profile in kidneys from rat treated by 40mg/kg AA I for 2-6 days. miRNAs with significantly different expression of global miRNA expression profile were validated by qRT-PCR. For miRNAs still significantly disregulation, we further examined the expression in plasma of rats treated with AAI dosed at 10, 20 and 40mg/kg AAI for 2-6 days by qRT-PCR. miRNAs with significantly dysregulation in plasma, their expressionin brain, liver and heart was examined for kicking out the non-specific disregulation in AAI induced acute kidney injury, so that the significant dysregulation miRNAs with specificity in kidney and plasma was found as potential biomarkers for AAI induced acute kidney injury.本研究旨在筛选马兜铃酸I（AAI）诱导急性肾损伤的潜在生物标志物。本研究采用微阵列分析技术，对给予40mg/kg剂量AAI、造模2-6天的大鼠肾脏组织中的微小RNA（miRNA）表达谱进行检测。对全局miRNA表达谱中差异表达显著的miRNA，采用实时定量PCR（qRT-PCR）进行验证。对于经验证仍存在显著表达失调的miRNA，本研究进一步采用qRT-PCR检测其在给予10、20、40mg/kg剂量AAI、造模2-6天的大鼠血浆中的表达水平。针对血浆中存在显著表达失调的miRNA，本研究进一步检测其在大鼠脑、肝、心脏组织中的表达水平，以排除AAI诱导急性肾损伤过程中非特异性的表达失调现象，最终筛选出在肾脏与血浆中均存在特异性表达失调的miRNA，作为AAI诱导急性肾损伤的潜在生物标志物。