Paternal methamphetamine exposure differentially affects first and second generations in mice

To determine whether paternal methamphetamine exposure alters the development, behavior and gene expression of first (F1) and second (F2) generations. We administrated methamphetamine (5 mg/kg) or saline (10 ml/kg) to sire (F0) and performed gene expression profiling analysis using data obtained from RNA-seq of striatum of 7-week-old male F1 mice. Male F1 mice were mated with untreated female mice to obtain F2 mice. Male F1 mice were mated with female mice to obtain F2 mice. RNA-seq of striatum of 7-week-old male F2 mice was also analyzed in the same way. Comparative gene expression profiling analysis of 7-week-old striatum RNA-seq data from male F1 mice born to methamphetamine or saline-treated sires. The same analysis of male F2 mice obtained by mating male F1 mice with untreated females.