Triticum turgidum strain:Kronos Transcriptome or Gene expression

For this project, we have sequenced, assembled and annotated a transcriptome of a tetraploid wheat T. turgidum cv. Kronos. The sequencing libraries were prepared from root and shoot tissues from young 2-3 week old seedlings, developing spikes at the booting stage and grain 20 days post anthesis. An additional library from multiple tissues was normalized with double-stranded DNA nuclease. All sequencing was carried out on the Illumina HiSeq platform using the 100 bp paired-end protocol (562.4 million reads). The assembly was constructed using a multiple k-mer approach with a de novo assembly algorithm implemented in CLC Genomics Workbench 5.5 and additional redundancy reduction with CD-HIT and blast2cap3 programs. Open reading frames and proteins were predicted using BLASTX searches and a findorf algorithm.

在本项目中，我们已对四倍体小麦T. turgidum cv. Kronos的转录组进行了测序、组装和注释。测序文库由幼苗根和茎组织制备，幼苗生长至2-3周，正处于拔节阶段和授粉后20天的穗期。此外，从多个组织中制备的文库经过双链DNA核酸酶标准化处理。所有测序工作均在Illumina HiSeq平台上，采用100碱基配对末端协议（共计562.4百万个reads）进行。组装采用多种k-mer方法构建，并在CLC Genomics Workbench 5.5中实现了从头组装算法，并通过CD-HIT和blast2cap3程序进一步减少了冗余。通过BLASTX搜索和findorf算法预测开放阅读框及蛋白质。

搜集汇总

背景与挑战

背景概述

该数据集包含四倍体小麦T. turgidum cv. Kronos的转录组测序数据，涵盖了根、茎、穗和籽粒等多个组织的基因表达信息。测序使用Illumina HiSeq平台进行，采用100 bp双端测序协议，共获得5.624亿条读长，并通过多种生物信息学方法进行组装和注释。