The scRNA-seq datas of foreskin mesenchymal stromal cells and human umbilical cord mesenchymal stromal cells
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https://data.mendeley.com/datasets/f4b2ykfv56
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资源简介:
First, we digested the 3rd passage of foreskin mesenchymal stromal cells (FSMSCs) and human umbilical cord mesenchymal stromal cells (HuMSCs) with the cell survival rate higher than 80%. Second, the concentration of cell suspension were adjusted to 1×106 cells/ml for 10X Genome Single Cell Platform (Single Cell 3’ library and Gel Bead Kit V3). Then, the samples were processed as follows: GEMs creation, thermal cycling, post cycling cleanup, cDNA amplication, library preparation, library quantification and sequencing on Illumina Hiseq3000. Finally, the result of sequencing was mapped to GRCh38 human genome, and reads were counted by cellranger software (version 3.1.0).
创建时间:
2022-03-25



