Streptomyces ambofaciens time resolved transcriptome

In this study we have applied an integrated system biology approach to characterize the metabolic landscape of Streptomyces ambofaciens and to identify a list of potential metabolic engineering targets for the overproduction of the secondary metabolites in this microorganism. We focused on an often overlooked growth period (i.e. post-exponential phase) and, by integrating constraint-based metabolic modelling with time resolved RNA-seq data we depicted the main effects of changes in gene expression on the overall metabolic reprogramming occurring in S. ambofaciens. Moreover, through metabolic modelling, we identified a set of candidate overexpression gene targets hypothetically leading to spiramycin over-production.