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Quantitative real–time RT-PCR analysis of total RNA encoding the kynurenine pathway enzymes in mouse and human MSCs cultured in the absence or presence of IFN-γ (100 IU/ml) or IFN-β (2,000 IU/ml) for 72 hours.

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Figshare2011-02-16 更新2026-04-29 收录
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https://figshare.com/articles/dataset/Quantitative_real_time_RT_PCR_analysis_of_total_RNA_encoding_the_kynurenine_pathway_enzymes_in_mouse_and_human_MSCs_cultured_in_the_absence_or_presence_of_IFN_100_IU_ml_or_IFN_2_000_IU_ml_for_72_hours_/468722
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The gene/β-actin ratios were multiplied by 10,000 for clarity purposes. Data are mean ± standard deviation (SD). Abbreviations: IFN-γ, interferon-γ; IFN-β, interferon-β; MSCs, mesenchymal stem cells; PBMCs, peripheral blood mononuclear cells; IDO1 and IDO2, indoleamine 2,3-dioxygenase 1 and 2; KYNU, kynureninase; ACDMS, aminocarboxymuconate-semialdehyde decarboxylase; TPH1 and TPH2, tryptophan 5-monooxygenase 1 and 2; KMO, kynurenine hydroxylase; HAAO, 3-hydroxyanthranilate 3,4 dioxygenase; CCBL1 and AADAT, kynurenine aminotransferases I and II respectively; AFMID, kynurenine formamidase; QPRT, quinolinate phosphoribosyltransferase; TDO2, tryptophan 2,3-dioxygenase.
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2011-02-16
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