Transcription profiling of mouse Pax6 heterozygous and wildtype newborn mouse lenses

This is an integrative genome-wide approach to identify downstream networks controlled by Pax6 during mouse lens and forebrain development. Differential gene expression was analyzed in Pax6 mouse heterozygous and wildtype newborn mouse lenses, with subsequent comparison of this data with Pax6 forebrain expression data (Holm et al., 2007). Experiment Overall Design: Three biological replicate experiments were performed from wildtype and heterozygote mice.本研究采用整合式全基因组分析策略，旨在鉴定小鼠晶状体与前脑发育过程中由配对盒基因6（Pax6）调控的下游基因调控网络。研究分析了Pax6杂合与野生型新生小鼠晶状体的差异基因表达谱，并将该数据与Holm等人2007年发表的Pax6前脑表达数据集进行了比对。实验整体设计：针对野生型与Pax6杂合小鼠，开展了三次生物学重复实验。