The developmental transcriptome of Aedes aegypti early embryos

The study aims at understanding transcriptional dynamics occurring during the early embryonic development of the yellow fever mosquito Aedes aegypti. Duplicate embryo samples were collected at four discrete intervals after egg laying (at 1 h, 3 h, 5 h, and 7 h) and immediately crushed in Trizol. Following total RNA isolation with on-column DNase treatment using PureLink (Ambion) kit, and library construction, the RNA samples were sequenced from both ends using Illumina NovaSeq6000 platform.