Expression data from U937 monocytes treated with LysoPPC and FSL-1

Monocytes and monocyte derived macrophages, besides natural killer (NK) cells, are crucial for elimination of senescent cells. There is evidence that TLR2/6 together with the lipid receptor CD36 may be the pattern recognition receptor that are most involved in the sensing of epitopes connected to cellular aging, senescence and severe cellular distress. Additionally, the interplay of these scavenger receptors is required for uptake of TLR2/6 agonist FSL-1. Therefore, we investigated whether lysophospholipids would induce or interfere with (TLR2/6 mediated) gene regulation in U937 monocytes. Cells were or were not pre-treated with lysophospholipids (10µM) for 1 hour and then stimulated with the TLR2/6 ligand (1µg/ml) for 2 hours. The human myeloid leukemia cell line U937 was exposed to 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocoline (LysoPPC) and FSL-1 This study was performed in biological quadruplicates for human myeloid leukemia cell line U937