Expression data from adipose tissue macrophages of mice with or without moderate aerobic exercise

ATMs, which can constitute up to 50% of the cells in adipose tissue of obese mice 5, play a pivotal role in both metabolic and immune regulation of adipose tissue. This is particularly evident in both healthy states and in conditions associated with metabolic syndrome. To delve into the precise mechanisms of the ATMs in response to exercise, we utilized exercise-induced primary ATMs and miRNAs microarray to investigate the effects of an 8-week aerobic exercise training on ATMs. Following the exercise regimen, ATMs were sorted using flow cytometer and subsequently analyzed through bioinformatics analysis to identify changes in miRNA expression. C57BL/6 mice were randomly assigned to either a sedentary (S) or an exercise (E) group. The S group remained inactive, while the E group underwent a one-week treadmill adaptation, followed by an 8-week aerobic treadmill exercise protocol (60 min/ day, 5 days/ week, at 65%-75% VO2max). Post-training, to investigate the effects of exercise in ATMs at the molecular level, miRNA microarray analysis was performed, followed by bioinformatic analysis.