Molecular characterisation of ribonucleic acid (RNA) interference in Fusarium circinatum

Identification and inference of the evolutionary relationship of seven known Ribonucleic acid (RNA) interference components Fusarium circinatum, its closely related species as well as other fungi outside of the genus. Twenty-one Fusarium species and thirteen species from other genera were chosen. The genes of interest include Argonaute, Dicer, RNA-dependent RNA polymerase, Replication protein A1 and A2, Mitochondrial ribosomal protein L3 (MRPL3) and Quelling Deficient-2 interating protein (QIP). A proof of concept of the functionality of RNA interference (RNAi) in Fusarium circinatum. These was done by applying double-stranded RNA targeting a fluorescence gene in Fusarium circinatum.

本数据集旨在识别和推断已知七种拟南芥（Fusarium circinatum）RNA干扰（RNAi）成分的进化关系，包括其近缘种及其他非属拟南芥属的真菌。选取了二十一种种拟南芥和十三种其他属的真菌。所关注基因包括Argonaute、Dicer、RNA依赖性RNA聚合酶、复制蛋白A1和A2、线粒体核糖体蛋白L3（MRPL3）以及沉默缺陷-2相互作用蛋白（QIP）。数据集验证了RNA干扰在拟南芥中的功能，通过靶向拟南芥中的荧光基因的双链RNA进行验证。