ATAC-Seq of human early thymopoieisis

Four sorted human thymic subpopulations (CD34+1a-7+, CD34+1a+, EC, LC) from two donors were used for ATAC-Seq. Samples were sequenced in house in single-end 75nt mode using the NextSeq® 500/550 (Illumina) according to manufacturer's instructions. Reads were trimmed with sickle and aligned with bowtie2 using default settings. Aligned reads from tCD34+1a-7+ and tCD34+1a+ populations from the two donors were merged to generate the tCD34 ATAC sample. Overall design: Chromatin accessibility using ATAC-Seq was examinated for four human thymic subpopulations (CD34+1a-7+, CD34+1a+, EC, LC) from two donors.