TEMPORAL STABILITY IN AGRICULTURAL AND URBAN FULL-SCALE ANAEROBIC DIGESTERS

P. Dabert, J. Buffet, S. Le Roux, M. Daumoin, P. Saint Cast, et al.. Temporal Stability in Agricultural And Urban Full-Scale Anaerobic Digesters. Orbit 2016, Organic Resources and Biological Treatment, 10 th International Conference on “Circular Economy and Organic Waste”, May 2016, Heraklion, Greece. pp.8. ?hal-02604657? The aim of this work was to study five full-scale digestion plants over the course of a year to analyse the influence of the type of substrate treated and type of process used on the digestate composition and microbial community structure in real working conditions. The studied plants were two agricultural, one centralized, and two urban digesters working under mesophilic and thermophilic conditions to treat both solid and liquid wastes. Sampling was conducted every 2 months. Digestate physico-chemical properties and microbiological compositions were studied using classical organic matter analyses (dry matter content, organic matter content, total carbon, total nitrogen, organic nitrogen, ammonia-nitrogen, and nitrous-nitrogen)and molecular microbiology techniques (16S rDNA-targeted quantitative PCR, molecular typing and Illumina MiSeq Next Generation Sequencing)