Thermodynamic parameters of Fab interactions with VEGF and HER2.

ΔΗPBS and ΔΗTris were determined by ITC in PBS (pH 7.4) or Tris (pH 7.5) buffer at 30°C.
ΔΗCORR, corrected ΔΗ, was calculated as described in Results.
ΔSTOT, net total ΔS, was calculated from ΔΗCORR and ΔG as described in Methods.
ΔCp, heat capacity change, was calculated by linear regression of the temperature versus ΔH (Figure S6 and Methods). ΔCp of Herceptin/HER2 (−370 calK−1 mol−1+/−30 calK−1 mol−1) is based on [26]. Standard errors of ΔCp measurements for bH1-44/VEGF and bH1-44/HER2 interactions are 7 and 13 calK−1 mol−1, respectively.
ΔSSOLV = ΔCpln(T/Ts*), where T = 303.15 K and Ts* = 385.15 K.
ΔSRT was estimated to −8 cal K−1 mol−1 for a protein binding reaction.
ΔSCONF was calculated as ΔSCONF = ΔSTOT−ΔSSOLV−ΔSRT, see Methods.
Standard errors of the ITC measurements were generally within 10% (See Figure 4).