Chromatin accessibility analysis of human monocyte derived macrophages (hMDM) in response to H2O2 or DMNQ stimulation

Atherosclerosis is closely associated with oxidative stress, characterized by the generation of reactive oxygen species (ROS). ROS can serve as a polarization queue depending on localization. We observed that predominantly foam cells elicit high mitochondrial ROS burden in human and mouse atherosclerotic lesions. Our results show changes in chromatin accessibility with DMNQ, a mitochondrial-specific ROS inducer, compared to H202 treatment for induction of general ROS. Human monocyte-derived macrophages (hMDM) chromatin of untreated, as well as H2O2 or DMNQ. Monocyte-to-macrophage differentiation was carried out with M-CSF (macrophage-colony stimulating factor) in presence of H2O2 or DMNQ for 7 days and then cells have been seeded without M-CSF in presence of H2O2 or DMNQ for 24h. Data were generated by deep sequencing, with cells deriving from four indepedent donors, using Illumina sequencing.