Molecular mechanism for the synchronized electrostatic coacervation and co-aggregation of alpha-synuclein and tau

The following metadata refers exclusively to electron paramagnetic resonance (EPR) measurements, which represent the contribution of the PARACAT students to this work

Data type: EPR spectroscopic measurements and simulations

Files are in .DTA, .DSC, .m, .mat, and .xlxs, formats

Information on origin of the data:

EPR spectroscopic measurements in .DTA and .DSC formats

EPR spectroscopic simulation and analyses in .m and .mat format

“Ready-to-plot”, processed EPR spectra are in .xlxs format.

The data are generated by:

CW-EPR measurements were performed with a Bruker ELEXSYS E580 X-band spectrometer equipped with a Bruker ER4118 SPT-N1 resonator operating at a microwave (MW) frequency of ∼9.7 GHz. The temperature was set to 25 °C and controlled by a liquid nitrogen cryostat.

If the dataset includes multiple files that relate to each other:

Files in PARACAT_WP3_20221219_EPR folder includes EPR spectroscopic measurements and computer simulations/analyses, original data are in  .DTA/.DSC formats; files in .m format were used to process the data.

NB. See the “READ ME” text file for more detailed information on files organization.

Information on:

Abbreviations:

avg = averaged

aS_24 = alpha-synuclein protein with TEMPOL spin label at position 24 of the polypeptidic chain

aS_122 = alpha-synuclein protein with TEMPOL spin label at position 122 of the polypeptidic chain

pLK = poly-lysine

Tau441 = Tau protein with complete amino-acid sequence

Tau_DNt = truncated Tau protein lacking N-terminal (see paper methods for further details)

Units of measurement:

Temperature: °C (Celsius)

Microwave Frequency: GHz (Giga-Hertz), MHz (Mega-Hertz), kHz (kilo-Hertz)

Microwave Power: mW (milli-Watt)

Magnetic Field: mT (milli-Tesla)

Time: s (seconds)

Concentration: μM (micro-Molar), % w/v (percentage weight-volume)