Transcriptional responses of GII.4 human norovirus infection in jejunal human intestinal enteroids

The goal of this project was to evaluate intestinal epithelial responses to GII.4 human norovirus infection. Overall design: Jejunal (J2 and J11) human intestinal enteroid cultures infected with GII.4 human norovirus. Mock- and gamma-irradiated GII.4 (gGII.4) inoculated cultures were used as controls. We performed RNA sequencing (RNA-Seq) to determine transcriptional responses to GII.4 at 6, 10 and 24 hours post-inoculation (hpi) in comparison to mock-inoculated cultures (3 hpi) or inoculation with gGII.4 HuNoV (6, 10 and 24 hpi).