Genome-wide Ezh2 and Tcf1 occupance in follicular helper T cells

mapping Ezh2 and Tcf1 binding sites in follicular helper T cells Overall design: C57BL/6 mice were then infected with vaccine virus, and 8 days later, CD44-high, CD62L-negative CD4+ T cells were further stained, and CXCR5+, SLAM-low follicular helper T cells were sorted and used for ChIPseq of Tcf1 or Ezh2. For negative control, CD62L+CD44-low naïve CD4+ T cells were sorted from CD4-Cre, Tcf1 fl/fl mice, or from CD4-Cre, Ezh2 fl/fl mice as Tcf1-deficient, or Ezh2-deficient naive CD4+ T cells, respectively.