Cholesterol and TNF-induced transcriptomic changes in Mus musculus aortic smooth muscle cells. Cholesterol and TNF-induced transcriptomic changes in Mus musculus aortic smooth muscle cells

To map gene regulation downstream of cholesterol overload and NF-kappaB signaling in smooth muscle cells (SMCs), we cultured primary aortic SMCs from wildtype mice with cyclodextrin-complexed cholesterol or the prototypical NF-kappaB activator, tumor necrosis factor (TNF), or both.