Transcriptomic alterations in PBMCs of Long COVID patients

To further investigate the involvement of immune cells in acute and long-COVID-19 we use single-cell RNA sequencing (scRNA-seq) for characterization of peripheral blood mononuclear cells (PBMCs) from a select cohort of patients at the several time points of acute COVID-19 infection and 18 months post-acute infection. ScRNA-seq allows the identification of individual cells, classification of subsets and analysis of pathways involved, that may lead to possible answers regarding the pathophysiological principles of long-COVID. In the present study we aim to unravel the intricate details of immune cell alterations and the pathways involved in immune system perturbations in acute and chronic COVID-19 conditions.