Molecular roadmap of the healthy stress response in the mouse hippocampus (single-nucleus)

The acute stress response mobilizes energy to meet situational demands and re-establish homeostasis. Here, we used a brief forced swim exposure to trigger a strong stress response in mice. Using multiomic profiling we characterize the stress-induced molecular events in the dorsal (dHC) and ventral (vHC) hippocampus over time, across molecular scales, and down to the level of cell types and single cells. Here, we used a brief (6min) forced swim exposure and profiled the hippocampus using single-nucleus RNA-seq 45min after initiation of the experiment.急性应激反应可调动能量以适配情境需求并重建内稳态（homeostasis）。本研究采用短暂强迫游泳刺激，在小鼠体内诱发强烈的应激反应；借助多组学谱分析（multiomic profiling），我们对小鼠背侧海马（dorsal hippocampus, dHC）与腹侧海马（ventral hippocampus, vHC）中应激诱导的分子事件，开展了时序维度、多分子尺度直至细胞类型与单细胞层面的表征解析。本实验使用时长为6分钟的短暂强迫游泳刺激，并在实验启动后45分钟，通过单细胞核RNA测序（single-nucleus RNA-seq）对海马组织进行了组学谱分析。