Genome-wide location analysis of Abf1 and Reb1 in Saccharomyces cerevisiae

Abf1 and Reb1, two general regulatory factors playing roles at promoters and other genome functional sites in budding yeast, were mapped genome-wide by ChIP-sequencing using strains expressing TAP-tagged versions of the proteins. As expected on the basis of previous in silico analysis of promoter regions, we found that these factors are enriched at the promoters of ribosome biogenesis (Ribi) genes, a large regulon of more than 200 genes required for ribosome biosynthesis and assembly, and known to be coordinately regulated in response to nutrient availability and cellular growth rate.Abf1与Reb1为两种通用调控因子，可在出芽酵母的启动子及其他基因组功能位点发挥调控功能。本研究采用表达该两类蛋白TAP标签融合版本的菌株，通过ChIP测序（ChIP-sequencing）在全基因组范围内对二者完成了定位分析。基于此前针对启动子区域开展的计算机模拟分析（in silico analysis），本研究得到了与预期相符的结果：两类因子在核糖体生物发生（Ribi）基因的启动子区域显著富集。Ribi基因属于一类大型调控子（regulon），涵盖200余个参与核糖体生物合成与组装的基因，且已知其会根据营养可获得性与细胞生长速率进行协同调控。