Development of gene expression signatures for the A549 cells that overexpressed of FOSB. Development of gene expression signatures for the A549 cells that overexpressed of FOSB

Strongly induction of FOSB was observed in a cationic anticancer peptide TP4 treated human A549 lung cancer cell-line, causing obvious cell death. This work aims to address the gene expression profiling upon FOSB overexpression in A549 cell. Overall design: A549 cells were transfected with either FOSB-GFP or GFP vector for 40hr. Duplicated total RNA samples (i.e. two GFP and two FOSB-GFP transfected groupse) were collected for further microarray study.