RNA-seq to identify differentially expressed mRNAs after cKO of Ybx1 in DRG [RNA-seq]

To identify the target mRNAs of Ybx1 in mouse DRG, we first performed RNAseq to screen differentially expressed mRNAs after knockout of Ybx1. We generated Wnt1-cre+/-;Ybx1fl/fl cKO mice to specifically knockout Ybx1 in DRG. Then we collected E13.5 DRG from cKO mice and litermate Ybx1fl/fl control mice. After RNA extraction and library construction, RNA sequcencing was performed. Finally, we identified 1638 differentially expressed mRNAs. This study provides a gene list which shows mRNA that can be regulated by Ybx1 in mouse DRG. Overall design: To identify target mRNAs of Ybx1 in mouse DRG, we generated Wnt1-cre+/-;Ybx1fl/fl cKO mice to specifically knockout Ybx1 in DRGs. Then we collected E13.5 DRG from cKO mice and litermate Ybx1fl/fl control mice. Each group has three biological male samples.