Hepatocyte‑specific Ccnd1 knockout in aged mice reduces inflammatory signaling

We performed hepatocyte‑specific knockout of Ccnd1 in 17‑month‑old C57BL/6J mice using AAV8‑TBG‑saCas9 with Ccnd1‑targeting sgRNA versus Rosa26 control. After 3 weeks, livers were harvested for bulk RNA‑seq to assess effects of elevated Ccnd1 with age. Upon receipt, mice were rested for ≥2 weeks and maintained on normal chow. At 17 months, mice received retro‑orbital AAV8‑TBG‑saCas9–U6-sgCcnd1 (3×10¹¹ vg/mouse) or Rosa26 control; livers (m‑lobe) were harvested 3 weeks later for bulk RNA‑seq.