Impact of BHLHE40-AS1 over expression in MCF10A cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE136579
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BHLHE40-AS1 was cloned into pBABE-Puro. Empty vector and pBABE-BHLHE40-AS1 were used to generate retrovirus and infect MCF10A cells. Transduced cells were selected with 1ug/mL puromycin for 2 weeks. 2x10^5 MCF10A-pBABE and MCF10A-BHLHE40-AS1 cells were plated in a 6 well dish. Total RNA was extracted 48 hours post plating. Three independent biological replicates were collected to check reproducibility.
创建时间:
2020-06-10



