Pharmacological Repositioning of the transcription factor PU.1 [CUT&TAG; CLICKonCUT&TAG]

This study aims to investigate the effects of PU.1 binding-site inhibitors/redistributors (DB2115, DB2373, DB2750, DB2826) upon the genomic occupancy of PU.1 and to understand DB2115-binding site preference amongst the PU.1 cistrome. MOLM-13, THP-1, MV411 and HL60 cell lines were treated with 5uM of DB compound for 12hr before cells were harvested for PU.1 CUT&Tag. "CLICKonCUT&TAG" was also performed on the PU.1 CUT&Tag samples to identify drug binding sites. Additionally, CRISPRd was utilized to block a specific PU.1 binding site (near the STRAP gene).