five

Impact of tRNA-Glu(UUC) and tRNA-Arg(CCG) on mRNA stability

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE77349
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Stable expression of tRNA-Glu(UUC) and tRNA-Arg(CCG) followed by whole-genome transcript stability measurements using a-amanitin mediated inhibition of RNA Pol II. Transfer RNA loci chr1.trna5 (Glu-TTC) and chr16.trna1 (Arg-CCG) were stably over-expressed in MDA-MB-231 cells. Cells were treated with a-amanitin (10ug/mL) and samples were collected at 0- and 9-nr. The RNA samples were then profiled.
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2018-08-13
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