Tmem161a is an important regulator for bone formation and bone strength through P38 MAPK pathway. Tmem161a is an important regulator for bone formation and bone strength through P38 MAPK pathway

Bone remoding is an extraordinarily complex process involving a variety of factors, such as genetic, metabolic and environmental impacts. Among them, genetic factor plays a major role, but not all have been identified. We investigated the role of Transmembrane 161a (Tmem161a) in bone structure and function using wild-type (WT) and Tmem161a depleted (Tmem161aGT/GT) mice. Overall design: Microarray analysis was used to screen for genes that are related to Tmem161a expression. RNA was isolated from WT and Tmem161a-KO cells (n = 3 each), and cRNA was prepared. cRNA from each sample was hybridized to a pre-equilibrated Clariom S mouse. microarray chip, which was then washed, stained, and scanned in an HP ChipScanner (Affymetrix Inc., Santa Clara, USA).