Raw data for: Epigenetic Age and Long-Term Cancer Risk Following a Stroke

In this study, we investigated whether stroke patients who later developed cancer exhibited an accelerated epigenetic age compared to those who did not. A total of 648 stroke patients were followed for 15 years, during which 83 individuals were diagnosed with cancer. Biological age (B-age) was estimated from DNA methylation data derived from whole blood samples collected within 24 hours of stroke onset. These samples were analyzed using the 450K or EPIC Illumina Beadchip arrays. We employed several epigenetic clocks to calculate B-age, including the Hannum, Horvath, PhenoAge, ZhangBLUP, ZhangEN, and the mitotic epiTOC clocks. After adjusting for multiple testing, competing risks, and key confounders, we found that patients who developed cancer were biologically older, as measured by the Hannum clock. This repository provides the raw idat files and processed DNA methylation values. We studied whether biological age, as obtained from DNA methylation, was associated with incidence of cancer in patients with stroke. To that aim, we followed-up a cohort of 648 patients with stroke within 15 years, of whom 83 developed cancer. DNA was extracted from whole peripheral blood collected in 10mL EDTA tubes during the first 24h after stroke onset. DNA methylation was obtained in two different batches. In one (n=410) we measured DNA methylation via the Human Methylation 450K Beadchip (Illumina, Netherlands, Eindhoven) in two different technical runs, while in the second batch (N=238) we used the Infinium Methylation EPIC Beadchip (Illumina, Netherlands, Eindhoven).