Metagenomic sequencing of wastewater microbial communities under nanoplastic and shear stress exposure

This project aims to investigate the effects of nanoplastic exposure and fluid shear stress on microbial community structure and antibiotic resistance gene (ARG) dissemination in wastewater environments using high-throughput metagenomic sequencing. Wastewater samples were subjected to different concentrations of polystyrene nanoparticles and variable shaking speeds to simulate environmental shear forces. The metagenomic datasets generated in this project provide a comprehensive resource for analyzing shifts in microbial composition, metabolic pathways, and ARG profiles under combined chemical and physical stressors, facilitating ecological risk assessment of emerging pollutants in aquatic systems.