Domains within the N-terminus of RqlI counter the toxicity of RqlH, independent of the putative HTH domain.

The schematics on the left indicate the locations of predicted domains and homology regions within RqlI and various truncation mutants. The table summarizes the effects of each RqlI expression construct on F11ΔrqlI titers recovered from 24 h cultures grown microaerobically. Whether or not expression of a given RqlI variant significantly increased bacterial titers in comparison to the empty vector control is indicated under “Microaerobic Rescue”. The “RqlH Inhibition” column indicates if a given RqlI variant could counter the toxic effects of RqlH when expressed in MG1655 grown for 6 h under aerobic conditions. See S10 Fig for full datasets.